Serotonin transporter transgenic sert cre mouse line. Transgenic mice inducible gene knockout somatic mutagenesis creer recombinase tamoxifen in utero rosa26 r26r xgal staining mouse models of human disease. A transgenic mouse model for monitoring oxidative stress. Usually these animals are maintained in germ free, gnotobiotic, or specificpathogen free facilities. Creexpressing rodent strains are often referred to as drivers because they can mediate. Progress in the somatosensory field has been restricted by the limited number of genetic tools available to study gene function in peripheral sensory neurons. Generation of nkx25creer transgenic mice for inducible cre expression in developing hearts ranjbarvaziri s, park s, nguyen nb, gilmore wb, zhao p, ardehali r thursday, june 15, 2017. Vecadherincre mice jcrb laboratory animal resource. When these sim1cre mice are bred with mice containing a loxp flanked sequence of interest, cremediated recombination will result in deletion of the flanked sequences in sim1 expressing tissues including hypothalamus. It has been frequently reported that many cre transgenic mouse strains express cre recombinase in nontarget cells or tissues. We would like to welcome you to the latest version of the cre database which we hope you will find to be considerably more user friendly. Generation of nkx25creer transgenic mice for inducible cre. With advanced transgenic technologies, they can generate transgenic mice and knockout and knockin mice more efficiently.
Charles gerfen, gensat offers transgenic bac cre recombinase driver lines that allow for cellspecific gene manipulations in the central nervous system of the mouse. In these animals, a cre transgene is expressed under control of murine vav gene regulatory elements. In this study, we developed a spc cre ert2 mouse model, in which a tamoxifen inducible cre recombinase cre ert2 is under the control of the human surfactant protein c spc promoter. Charles gerfen, gensat offers transgenic baccre recombinase driver lines that allow for cellspecific gene manipulations in the central nervous system of the mouse. Here we generated a credriver mouse line that expresses crerecombinase from the locus of the sensory neuron specific gene advillin. The journal of biological chemistry q 1993 by the american society for biochemistry and molecular biology, inc. Your feed back is always welcome so please dont hesitate to. As such, sim1 cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a. A timedependent conditional knockout mouse defines an inducible animal model in which a gene of interest is floxed thus temporally controllable at a given timepoint in embryonic, postnatal or adult animals after an additional breeding step with a creert2 deleter mouse line, the conditional knockout is temporally triggered by external induceragents, most often small molecules such as.
Strain name gene name promoter construct type target status reference owner email. Although several cre loxpbased gene knockout mouse models have been generated for the study of gene function in alveolar epithelia in the lung, their applications are still limited. Sep 11, 2011 progress in the somatosensory field has been restricted by the limited number of genetic tools available to study gene function in peripheral sensory neurons. This strain is used for elucidation of role of vecadherin in the neovascularization of the myocardial infarction model 1.
Cd4cre transgenic taconic biosciences humanized mice. Cre production summary in collaboration with the intramural program of the national institute of mental health pi. Over the past three decades, genetic engineering in mice has become an essential tool in biomedical research, from early manipulations, wherein germline targeting allowed the generation of wholeanimal knockout or transgenic expression costantini and lacy, 1981, gordon et al. Somatically inducible cre lines are used extensively to study gene function. Bac clone rp24361c4 bacpac resources containing the itgax cd11c gene but lacking the 5 end of the adjacent itgam gene, was modified by. The model was created by a targeted mutation knock in of high affinity creer t2 creesr1 into the gtrosa26 locus on chromosome 6 in c57bl6ntac es cells. Efficient recombination in diverse tissues by a tamoxifen.
Spccreert2 transgenic mouse for temporal gene deletion. Indeed, the crelox system has numerous problems that can affect physiological parameters, and these are missed when the correct control strains are not used. The generation of conditional knockout mice is a multiplestep process, which involves mating the flox mutant mouse line essential exons of the gene of interest are flanked by two loxp sites and the creexpressing mouse line. Generation and characterization of an advillin cre driver. Although several creloxpbased gene knockout mouse models have been generated for the study of gene function in alveolar epithelia in the lung, their applications are still limited. The mouse possessing this gene locus is called as flox mouse or floxed mouse. When doxycycline was provided to the mice, cre was activated leading to deletion of. These mice express cre recombinase under the control of the human bestrophin 1 best promoter. To date, a huge numerous cre transgenic mouse lines have been established and being generated continuously. Protein qualitycontrol, especially the removal of proteins with aberrant structures, has an important role in maintaining the homeostasis of nondividing neural cells 1. Considerations for the use of cre recombinase for conditional gene. As such, sim1cre transgenic mice may be useful in studying body weight homeostasis, obesity, leptin metabolism, or as a reporter strain for sim1transcription factor activity. Icr cd1 mouse model is of swiss origin and descended from the original two male and seven female albino noninbred mice, imported by the rockefeller institute in 1926 from lausanne, switzerland.
Generation of nsemercremer transgenic mice with tamoxifen. When these sim1 cre mice are bred with mice containing a loxpflanked sequence of interest, cre mediated recombination will result in deletion of the flanked sequences in sim1expressing tissues including hypothalamus. The number of iterations is therefore not such an issue in mice. Therefore, in postnatal and adult mice carrying nestin cre, cre is expressed throughout most of the central nervous system, eye, and also the kidneysthis was demonstrated by dubois and colleagues. Mosaic cre recombinase expression mrna is seen in 5090% of retinal pigment epithelium rpe cells, with some expression seen in the testis. A transgenic construct containing sequence encoding cre recombinase under the control of the mouse integrin alpha x cd11c promoter, and a bovine growth hormone poly a signal sequence, was introduced into b6cbaf1j donor eggs. The algorithm in mice is quite efficient and is surprisingly free of these issues because the filled missing values in the target variable arent used to fit the model in each iteration only those in the covariates. Inducible knockout mouse for timedependent gene control. This strain is currently unavailable due to replenishing of cryopreserved stocks please register interest. The tetracycline transactivator tta was placed under the control of 1.
Springer nature is developing a new tool to find and evaluate protocols. This strain represents an effective tool for generating retinal pigment epithelium rpe specifictargeted mutants that would be useful in studies of human retinopathies. Tomatoai14 mice had shown expressiinal cord and on in the whole sp surrounding. Pdf a nestincre transgenic mouse is insufficient for. However, a background level of spontaneous recombination due to unregulated expression of cre is particularly confounding for cancer models in which following the pathogenesis of the disease requires the introduction of sporadic mutations that are monitored over time.
May 15, 2009 the generation of conditional knockout mice is a multiplestep process, which involves mating the flox mutant mouse line essential exons of the gene of interest are flanked by two loxp sites and the cre expressing mouse line. In collaboration with the intramural program of the national institute of mental health pi. Transgenic mice protocol the science of transgenic. Descendants were distributed worldwide and the haicr was established in 1948 at the institute of cancer research. Cre fusions with an estrogen receptor mutant that only binds tamoxifen ert and a progesterone receptor that only binds ru486 have been developed and are being tested in mice.
Jci a mouse model of mycndriven retinoblastoma reveals. Metabolic pitfalls of cns crebased technology sciencedirect. Contractconditional knockout mouse producing service of trans genic. The cd4cre construct was created by replacing the the lck proximal promoter of a construct used to make a lckcre transgenic mouse taconic line 4197 with the mouse cd4 promoterenhancersilencer, which was provided by dr. Cre esr1 is expressed under the control of the gtrosa26sor gene. Generation of aggrecancreert2 knockin mice for inducible. The aim of our targeted transgenic service is to generate a single mouse line, where the only genetic modification is the change in the targeted locus. Generation of nkx25creer transgenic mice for inducible. Loss of autophagy in the central nervous system causes. The imsr is a searchable online database of mouse strains, stocks, and mutant es cell lines available worldwide, including inbred, mutant, and genetically engineered strains. Inducible gene targeting in mice using the crelox system brian sauer laboratory of biochemistry and metabolism, national institute of diabetes, digestive and kidney disease, national institutes of health, building 10, room 9n119, bethesda, maryland 208921800 email. Nkx25 is a homeoboxcontaining transcriptional regulator that serves as one of the earliest markers of cardiac lineage commitment. In this study, we developed a spccreert2 mouse model, in which a tamoxifeninducible cre recombinase creert2 is under the control of the human surfactant protein c spc promoter.
These mice displayed almost exclusive cremediated recombination in all peripheral sensory neurons. The 5flanking region of the rat synapsin i gene directs neuron specific and developmentally regulated reporter gene expression in transgenic mice. Anderson1,2,4 departments of 1cell biology, 3medicine, and 4ophthalmology, university of oklahoma health sciences center, oklahoma city. The conditional knockout method is also called as conditional gene disruption. Targeted expression of cre recombinase to cone photoreceptors in transgenic mice yunzheng le,1,2 john d. Production of conditional knockout micetransgenic inc. Prepare your model microbiome solutions and germfree mice. For detection of lacz expression tissues were fixed in icecold 2% formaldehyde0. Over the past few years many inducible andor tissuespecific cre mouse lines have been developed. Vecadherincre mice jcrb laboratory animal resource bank. Inducible gene targeting in mice using the cre lox system brian sauer laboratory of biochemistry and metabolism, national institute of diabetes, digestive and kidney disease, national institutes of health, building 10, room 9n119, bethesda, maryland 208921800 email. The target gene region is sandwiched by loxp, which is the cre recombinase target sequence. The national resource center for mutant mice of china nrcmm was established in 2001 for promoting the generation and sharing of mouse disease models.
Despite the increasing use of the crelox system, these issues were not visible to the scientific community previously and may have. Cggtrosa26sortm1rtta,egfpnagyj 005670 dow 2014 plos one pmid. Our targeted transgenic mouse projects now use gogermline, the revolutionary new technology to generate transgenic mice fast and efficiently. We have generated vavcre transgenic mice, which can be used to inactivate genes specifically in adult hematopoietic and endothelial cells. The crelox sitespecific recombination system has emerged as an important tool for the generation of conditional somatic mouse mutants. Targeted expression of cre recombinase to cone photoreceptors. Tremycnluc mice were provided by william weiss ucsf, san francisco, california, usa. Cre expressing rodent strains are often referred to as drivers because they can mediate. To investigate the role of camp response elementbinding protein creb in the adaptive responses to psychotropic drugs, we have developed inducible, brain regionspecific creb transgenic mice using the tetracyclineregulated gene expression system. The model was created by a targeted mutation knock in of high affinity creer t2 cre esr1 into the gtrosa26 locus on chromosome 6 in c57bl6ntac es cells. Characterization of cre mouse lines by means of tomato reporter. The transgene could be spatiotemporally induced by cre recombinase activity. Your other point i find very plausible, but i think its not.
To study the role of nkx25expressing progenitors at specific time points in cardiac development, we have generated a novel and inducible nkx25 mouse line by knocking in a creer cassette into the nkx25 genomic locus, while preserving the endogenous nkx25 gene. Characterization of cre mouse lines by means of tomato. Imputation mice in r still na left in dataset stack overflow. Jan 19, 2012 to induce oxidative stress for mice, asn 12. The cd4cre transgenic mouse was originally developed in the laboratory of dr. Inducible and brain regionspecific creb transgenic mice. Its possible to produce mice expressing lacz or gfp in the control of vecadherin promoter by mating between vecadherincre mouse and lacz reporter mouse or gfp reporter mouse carrying loxp sites. Elimination of drug resistant gene production of flox heterozygous mice optional, f1 heterozygous mice are crossed with cagflp mice or cagcre mice to elminate drug resistant gene. Specificity protein 2 sp2 is essential for mouse development and. Transgenic construct nsemercremer was constructed by linking the rat nse promoter 5. Here we generated a cre driver mouse line that expresses cre recombinase from the locus of the sensory neuron specific gene advillin. Generation of aggrecancreert2 knockin mice for inducible cre.
Transgenic mice expressing crerecombinase specifically in. Cre recombinase is expressed under the control of mouse cd4 regulatory elements. The creer t2 mouse was developed by artemis pharmaceuticals now taconic. Spccreert2 transgenic mouse for temporal gene deletion in. Jun 15, 2017 generation of nkx25creer transgenic mice for inducible cre expression in developing hearts. This method allows one to control gene activity in space and time in almost any tissue of the mouse, thus opening new avenues for studying gene function and for establishing sophisticated animal models of.
Creesr1 was generated by fusion of the cre bacteriophage p1 gene to the high affinity human estrogen receptor gene esr1. In addition to predominant expression in pv interneurons, we detected nav1. The rb flfl and p107 strains were provided by tyler jacks mit, cambridge, massachusetts, usa. Nestincre mice have a significant metabolic phenotype that is hard to discern from current literature. Therefore, in postnatal and adult mice carrying nestincre, cre is expressed throughout most of the central nervous system, eye, and also the kidneysthis was demonstrated by dubois and colleagues. Ranjbarvaziri s, park s, nguyen nb, gilmore wb, zhao p, ardehali r. Crossing a flox mouse with creexpressing mouse results in the deletion of. Embryonic reporter expression driven by sert cre mice. Jun 01, 2002 to investigate the role of camp response elementbinding protein creb in the adaptive responses to psychotropic drugs, we have developed inducible, brain regionspecific creb transgenic mice using the tetracyclineregulated gene expression system. To add inducibility to the crelox system, liganddependent chimeric cre recombinases, socalled creer recombinases, have been developed 36. Then next generation is crossed with wildtype mice with wildtype mice to remove flp or cre transgene. Colocalization of cre activity and rod bipolar cells on cross sections of retina from a 20. The cd4 cre construct was created by replacing the the lck proximal promoter of a construct used to make a lck cre transgenic mouse taconic line 4197 with the mouse cd4 promoterenhancersilencer, which was provided by dr.
Cre esr1 was generated by fusion of the cre bacteriophage p1 gene to the high affinity human estrogen receptor gene esr1. Clearly, key to successful conditional gene targeting is the availability of cre transgenic mouse strains in which cre activity is tightly controlled in space and time. Regulating rtta and target expression with cre teton krasg12d to model pancreatic cancer b6. Your feed back is always welcome so please dont hesitate to contact us with your suggestions. As pointed out by francis stewart, proximity to the waters of the rhine seems to be inportant for pursuit of this approach.
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